Merseyside and Cheshire Regional  Molecular Genetics Laboratory

Address

Merseyside and Cheshire Regional  Molecular Genetics Laboratory

Genetics Department

Liverpool Women’s NHS Foundation Trust

Crown St

Liverpool

L8 7SS

Contact 1

Mr Roger Mountford

Contact 2

Dr David Gokhale

Contact 3

 

Telephone 1

+44 (0)151 702 4219

Telephone 2

+44 (0)151 702 4225

Telephone 3

 

Fax

 

Email 1

Roger.Mountford@lwh.nhs.uk

Email 2

David.Gokhale@lwh.nhs.uk

Duty scientist

 dna.liverpool@nhs.net

Home page

http://www.lwh.me.uk/html/genetics.php

 

 

Services offered: 04/08/2010 update by Vicky Stinton

Disease

OMIM

UKGTN service*

Adrenoleukodystrophy (ALD -X-linked): ABCD1 gene sequencing & MLPA

300100

 YES

Aneuploidy: Trisomy 13 (Patau syndrome), Trisomy 18 (Edward syndrome),Trisomy 21 (Down syndrome)& XY aneuploidies e.g. Klinefeltor & Turner syndrome.

 

 

Breast/ovarian cancer : familial known BRCA1+BRCA2 mutations only.

113705

600185

 

Cerebral Autosomal Dominant Arteriopathy with Sub-cortical Infarcts and Leukoencephalopathy (CADASIL): NOTCH3 sequencing.

125310

YES

Charcot Marie Tooth disease/Hereditary Motor Sensory Neuropathy (CMT/HMSN): PMP22 duplication, PMP22 sequencing, MPZ sequencing, connexin 32 (GJB1) sequencing, mitofusin (MFN2) sequencing.

118200

YES

Colorectal cancer: KRAS codons 12, 13 & 61; BRAF V600E.    

Cystic Fibrosis (CF) - CFTR gene, Cystic Fibrosis Transmembrane Conductance Regulator: Common 32 CFTR mutations (Elucigene)

219700

YES

Dentatorubral-Pallidoluysian Atrophy (DRPLA): ATN1 triplet repeat analysis.

125370

YES

Duchenne Muscular Dystrophy (DMD)/Becker Muscular Dystrophy (BMD): Dystrophin exon copy number detection & linkage.

310200 300276

YES

Torsion dystonia - DYT1 deletion.

605204

YES

Familial Adenomatous Polyposis (FAP) coli: Known familial APC mutations only

175100

 

Familial Hypercholesterolaemia (FH): Common LDLR, APOB & PCSK 20 mutations. Sequencing LDLR exons 1-18, APOB exon 26 & PCSK9 exon 7. LDLR MLPA.    

Fetal Sexing

 

 

Fragile X disease (FraXA+FraXE):

FMR1 triplet repeat analysis.

309548, 309550

YES

Friedreich Ataxia (FRDA/FA) : Frataxin triplet repeat analysis & sequencing.

229300

YES

Gilbert syndrome

(Hyperbilirubinaemia I): UGT1A1 gene,

UDP-glucuronosyltransferase: UGT1A1

TA repeat analysis

143500

YES

Haemato-oncology: JAK2 V617F & exon 12 sequencing. FLT3 ITD; NPM1 ITD; B cell clonality.    
Hearing loss:Connexin 26 (Cx26): GJB2 sequencing; Connexin 30 (Cx30): common deletion; Mitochondrial m.1555A>G mutation (aminoglycoside induced deafness) 12100,604418  

Hereditary liability to pressure palsy (HLPP/HNPP): PMP22 deletion & PMP22 sequencing.

162500

 YES

Haemochromatosis(HCT/HH): HFE gene: c.845G>A; p.Cys282Tyr (C282Y) & c.187C>G; p.His63Asp (H63D) mutation analysis

235200

 YES

Hereditary Non Polyposis Colon Cancer (HNPCC): MLH1, MSH2 & MSH6 sequencing & MLPA; MSI analysis.

114500

 

Huntington's Disease (HD):

Huntingtin triplet repeat

expansion.

143100

YES

Lebers Hereditary Optic Neuropathy

(LHON): m.11778A>G,

m.3460A>G & m.14484T>C analysis.

535000

YES

Medium-chain acyl CoA dehydrogenase (MCADD): ACADM gene: c.985A>G; p.Lys329Glu (K329E) mutation analysis

201450

YES

Mitochondrial disorders

(MELAS,MERRF,NARP, Leigh

disease, Kearns Sayre, CPEO):

m.3243A>G,

m.8344A>G, m.8993T>C/G, large

scale rearrangements).

540000, 545000, 551500, 530000, 557000, 256000

YES

Myoclonus (Myoclonic) dystonia: SGCE  sequencing. 159900, 6041449  

Myotonic Dystrophy type I (DM1):

DMPK triplet repeat analysis.

160900

YES

Myotonic Dystrophy type 2 (DM2):

CNBP repeat analysis.

 

YES

Myotubular Myopathy: MTM1 sequencing. 310400  

Nail Patella Syndrome (NPS) -

LMX1B sequencing

161200

YES

Neuroferritinopathy (NFL) - FTL

exon 4 sequencing.

606159

YES

Oculopharyngeal Muscular dystophy (OPMD) - PABPN1 triplet repeat analysis 164300  
Pancreatitis (HP/PCTT) - hereditary: PRSS1 gene, Cationic Trypsinogen, TRY1: PRSS1 sequencing exons 2 & 3 & MLPA 167800 YES

Pancreatitis - idiopathic: SPINK1 gene, Serine Protease Inhibitor Kazal-type I: c.101A>G; p.Asn34Ser (N34S) mutation analysis

 167790

 

Pantothenate Kinase Associated

Neurodegeneration

(PKAN/Hallervorden

Spatz disease): PANK2 gene

sequencing

234200

 YES

Peutz-Jegher syndrome (PJS): STK11

(LKB1) gene sequencing & MLPA

175200

 YES

Prader Willi syndrome/ Angelman

syndrome (PWS/AS): methylation

studies & microsatellite analysis.

176270 / 105830

 YES

Rett syndrome: MECP2 sequencing + MLPA 300005  

Spinal and Bulbar Muscular

Atrophy

(SBMA): AR triplet repeat analysis

313200

 YES

Spinocerebellar ataxias (SCA1,

SCA2, SCA3, SCA6, SCA7, SCA17):

triplet repeat analysis.

164400, 183090, 109150, 183086, 164500,

607136

 YES

Spinal muscular atrophy (SMA):

SMN1 copy number & linkage analysis

253300

 YES

Uveal Melanoma: MLPA analysis    

Uniparental disomy – UPD7 (Russell-Silver) and UPD14 microsatellite analysis.

180860, 608149

 YES

Zygosity analysis

 

 YES

 

 

 

*Services offered outside main commissioning area geographical

boundaries under the UK specialist Genetic Testing Network - enquire

to the laboratory on costs.

 

*Services offered outside main commissioning area geographical

boundaries under the UK specialist Genetic Testing Network - enquire

to the laboratory on costs.